Title Restriction Enzymes: A History
Author Wil A. M. Loenen
ISBN 9781621821052
List price USD 49.00
Price outside India Available on Request
Original price
Binding Hardbound
No of pages 346
Book size 159 x 235 mm
Publishing year 2019
Original publisher Cold Spring Harbor Laboratory Press
Published in India by .
Exclusive distributors Viva Books Private Limited
Sales territory India, Sri Lanka, Bangladesh, Pakistan, Nepal, .
Status New Arrival
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Restriction enzymes cleave DNA at specific recognition sites and have many uses in molecular biology, genetics, and biotechnology. More than 4000 restriction enzymes are known today, of which more than 621 are commercially available, justifying their description by Nobel Prize winner Richard Roberts as “the workhorses of molecular biology.”

This book by Wil Loenen is the first full-length history of these invaluable tools, from their recognition in the 1950s to the flowering of their development in the 1970s and 1980s to their ubiquitous availability today. Loenen has worked with restriction enzymes throughout her career as a research scientist and came to know many of the leaders in this field personally and professionally. She is the author of several authoritative and widely appreciated reviews of the enzymes’ biology. This book was written with the close assistance of several of the field’s pioneers, including Rich Roberts, Stuart Linn, Tom Bickle, Steve Halford, and the late Joe Bertani. The seed for the book was sown at a retirement party for Noreen Murray, to whom the book is dedicated, and its roots lie in a remarkable 2013 conference at Cold Spring Harbor Laboratory that celebrated the people and events that were vital to the field’s development.

Funding for the book was made possible by the Genentech Center for the History of Molecular Biology and Biotechnology at Cold Spring Harbor Laboratory. A companion website to the book (www.restrictionenzymes.org) includes an electronic version of the text and ancillary material that is freely accessible to readers.





Chapter 1. Discovery of a Barrier to Infection and Host-Controlled Variation: 1952–1953

Chapter 2. Host-Controlled Variation Is Methylation and Restriction of DNA: The 1960s

Chapter 3. The Discovery of Type II Restriction Enzymes: The 1970s

Chapter 4. Expansion and Cloning Restriction Enzymes: The 1970s and Early 1980s

Chapter 5. The First Decade after the Discovery of EcoRI: Biochemistry and Sequence Analysis during the 1970s and Early 1980s

Chapter 6. Variety in Mechanisms and Structures of Restriction Enzymes: ~1982–1993

Chapter 7. Crystal Structures of Type II Restriction Enzymes and Discovery of the Common Core of the Catalytic Domain: ~1993-2004

Chapter 8. Improved Detection Methods, Single-Molecule Studies, and Whole-Genome Analyses Result in Novel Insights in Structures, Functions, and Applications of Type I, II, III, and IV Restriction Enzymes: ~2004–2016

Summary and Conclusions

Appendix A: The History of Restriction Enzymes October 19–21, 2013 Meeting Program

Appendix B: Modern-Day Applications of Restriction Enzymes


About the Author

Target Audience:

People interested in Biotechnology; Molecular Biology; Genetics; History of Science.

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